Clarification of Tomato Juice with Polygalacturonase Obtained from Tomato Fruits Infected by Aspergillus niger
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چکیده
منابع مشابه
Extraction and clarification of apple juice with polygalacturonase obtained from apple (Malus domestica) fruits deteriorated by Aspergillus niger
Pectinase is used commercially in the clarification and extraction of fruit juice from different fruits. Green apples and Red apples obtained from the fruits section of a supermarket, Idiroko road, Ota were surface sterilized and inoculated with Aspergillus niger. The stock culture was subcultured on Sabouraud Dextrose agar plates and 72-hr-old culture of Aspergillus niger served as the inoculu...
متن کاملCharacterisation of Partially Purified Cell Wall-degrading Enzymes: Polygalacturonase and Cellulase from Tomato Fruits Degraded by Aspergillus Niger
Aspergillus niger is a soil saprobe and produces a wide array of hydrolytic and oxidative enzymes and cell wall degrading enzymes. An investigation on the various properties of partially purified polygalacturonase and cellulase enzymes extracted from tomato fruits deteriorated by Aspergillus niger was carried out in this study. The results obtained shows that temperature, pH and substrate conce...
متن کاملProduction, purification and biochemical characterization of an exo-polygalacturonase from Aspergillus niger MTCC 478 suitable for clarification of orange juice
Polygalacturonases (PG) represent an important member of pectinases group of enzymes with immense industrial applications. A fungal strain Aspergillus niger MTCC478 was used for the production of polygalacturonase both under submerged and solid-state fermentation condition. Further its production was optimized under solid-state fermentation condition with media comprising of wheat bran and tea ...
متن کاملMango juice clarification with polygalacturonase produced by Aspergillus awamori MTCC 9166 - Optimization of conditions
Pectin rich fruit juices need enzymatic treatment for clarification. As fruits are rich in pectin, pectinolytic treatment can efficiently reduce viscosity resulting in clarified fruit juice. Enzymatic treatment of mango pulp results in 80% recovery of total juice present in the fruit. Use of pectin degrading polygalacturonases increases both fruit juice extraction and clarification. Polygalactu...
متن کاملAn acidic pectin lyase from Aspergillus niger with favourable efficiency in fruit juice clarification.
The pectin lyase gene pnl-zj5a from Aspergillus niger ZJ5 was identified and expressed in Pichia pastoris. PNL-ZJ5A was purified by ultrafiltration, anion exchange and gel chromatography. The Km and Vmax values determined using citrus pectin were 0.66 mg ml(-1) and 32.6 μmol min(-1) mg(-1) , respectively. PNL-ZJ5A exhibited optimal activity at 43°C and retained activity over 25-50°C. PNL-ZJ5A w...
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ژورنال
عنوان ژورنال: Pakistan Journal of Biological Sciences
سال: 2015
ISSN: 1028-8880
DOI: 10.3923/pjbs.2015.74.80